Heterologous induction of a predicted promoter sequence for paraquat-inducible genes of Chromobacterium violaceum in response to paraquat compound.
نویسندگان
چکیده
aCentro de Ciências Agrárias, Universidade Federal do Vale do São Francisco – UNIVASF, Rodovia BR 407, Km 12, Projeto de Irrigação Nilo Coelho, CEP 56300-000, Petrolina, PE, Brazil bPontifícia Universidade Católica do Paraná – PUCPR, Rodovia BR 376, Km 14, Campus de Ciências Agrárias, Costeira, CEP 83010-500, São José dos Pinhais, PR, Brazil cLaboratório Nacional de Computação Científica – LNCC, Av. Getúlio Vargas, 333, Quitandinha, CEP 25651-075, Petrópolis, RJ, Brazil
منابع مشابه
Superoxide radical-generating compounds activate a predicted promoter site for paraquat-inducible genes of the Chromobacterium violaceum bacterium in a dose-dependent manner.
The purpose of the present study was to functionally evaluate the influence of superoxide radical-generating compounds on the heterologous induction of a predicted promoter region of open reading frames for paraquat-inducible genes (pqi genes) revealed during genome annotation analyses of the Chromobacterium violaceum bacterium. A 388-bp fragment corresponding to a pqi gene promoter of C. viola...
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Environmental bacteria are endowed with several regulatory systems that have potential applications in biotechnology. In this report, we characterize the arsenic biosensing features of the ars response system from Chromobacterium violaceum in the heterologous host Escherichia coli. We show that the native Pars/arsR system of C. violaceum outperforms the chromosomal ars copy of E. coli when expo...
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We report the first example of a gene, hmp, encoding a soluble flavohemoglobin in Escherichia coli K-12, which is up-regulated by paraquat in a SoxRS-independent manner. Unlike what is found for other paraquat-inducible genes, high concentrations of paraquat (200 microM) were required to increase the level of hmp expression, and maximal induction was observed only after 20 min of exposure to pa...
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ورودعنوان ژورنال:
- Brazilian journal of biology = Revista brasleira de biologia
دوره 75 2 شماره
صفحات -
تاریخ انتشار 2015